Application of PET media bottle in cell culture

The development of cell culture technology has been very mature. Cell growth needs to meet various requirements such as environment, temperature, osmotic pressure and pH value. In addition to providing basic nutrients in various synthetic media, it also needs to be based on different cells and different culture purposes. Add serum and other substances to promote cell growth. Therefore, the PET media bottle has a wide range of applications in the field of cell culture.

Serum is a complex mixture formed by removing fibrinogen from plasma. It mainly includes fetal bovine serum, calf serum, adult bovine serum, horse serum, sheep serum, chicken serum, rabbit serum, etc., among which fetal bovine serum The use of is the most common. Serum provides important substances such as extracellular matrix, growth factors, and transferrin. The proportion of serum added should be determined according to different cells and different research purposes. 10% to 20% of serum can maintain the rapid growth and proliferation of cells, which is called growth medium; in order to maintain the slow growth or immortality of cells, 2% to 5% of serum can be added, which is called maintenance medium.

PET storage media bottle

Due to the particularity of serum, its storage temperature should be between -5°C and -20°C. Serum bottles are generally made of transparent PET material processed by injection stretch blow technology. This material has good heat aging resistance, brittleness temperature is -70 ℃, and still has a certain degree of toughness at -30 ℃, which meets the storage requirements of serum . And the serum bottles will be sterilized, no pyrogen, no endotoxin, no DNase, no RNase and other factors that affect cell growth.

Serum is an indispensable item for cell culture. With the continuous development of biological products, immunotherapy, scientific research institutes and other fields, the demand for serum will also increase, and PET media serum bottles will also be used in the field of cell culture. More extensive applications.